Use of Pulsed Electric Field as a Low-Temperature and High-Performance “Green” Extraction Technique for the Recovery of High Added Value Compounds from Olive Leaves

Olive leaves (OLL), an agricultural waste by-product, are considered a significant bioresource of polyphenols, known as bioactive compounds. This study evaluates the pulsed electric field (PEF) technique for extraction polyphenols from OLL. The parameters included series “green” solvents (ethanol, water well mixtures them at 25% step gradient) and different input values pulse duration PEF. phytochemical degree was evaluated using total phenol concentration (Folin–Ciocalteu method) high-performance liquid chromatography (HPLC) analyses, while antioxidant activity assessed differential scanning calorimetry (DSC). results obtained PEF extracts were compared with those produced without application. highest effect observed aqueous ethanol, v/v, 10 ?s. increase in reached 31.85%, specific metabolites 265.67%. recovery found to depend on solvent, treatment structure extracted.